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What Is C18? A Guide on the Basics of HPLC Columns
HPLC, or high-performance liquid chromatography, has been around for more than 50 years. In that time, science has drastically improved the reliability and accessibility of this method. But how does HPLC work? How do you know which column to use? Do you know the…
How to Transfer HPLC Methods to UHPLC
High-performance liquid chromatography is an important analytical tool for a variety of research applications. One of the downfalls of this system is the time it takes to get results. What if we told you there was an HPLC system that generates the accurate results you…
Introducing a new UHPLC Series dedicated for UHPLC analysis.
Introducing a new UHPLC Series dedicating for UHPLC analysis. Complete solution with using one type of particle The Develosil UHPLC Series is designed for UHPLC analysis with using pure 1.6 µm silica particles. C30, C18, C8, C1 and HILIC functional groups…
How should you cook your eggs?
Eggs have been known to be a rich protein source as well as great source of various nutrients such as antioxidants. They are considered one of the most important sources of xanthophylls.[1] Xanthophylls, one of the major groups of carotenoids, have a potential…
A novel analytical procedure for assaying lysozymes
Lysozymes (EC. 3.2.1.17) are widely recognized for their contribution to the antibacterial defense in many animals and their use as a preservative in foods and pharmaceuticals. [1] The key characteristic for all lysozymes is their ability to hydrolyze the…
The Common Problems of HPLC Columns and How to Deal With It
Having troubles on HPLCs can be incredibly frustrating. You’re trying to get some analysis done, and your research can’t move forward until you figure out why your tools aren’t working. There are some common problems of HPLC columns that pop up from time to time….
The Types of (U)HPLC Columns and Applications Sold on the Market Today
When you’re trying to analyze what’s in a certain mixture, high-performance liquid chromatography is one of the best tools at your disposal. This method runs your mixture through a sort of filter to pull out different molecules that may be in your sample. From there…
Analysis of Vitamin E Compounds in Colored-Grain Wheat, Tritordeum, and Barley using HPLC
Vitamin E is a group of compounds that include tocopherols and tocotrienols. The structure of each compound in this family include a chromane double ring with a hydroxyl group and a hydrophobic side chain. The chromane double ring with the hydroxyl group can donate an…
Introduction to Buffer Solutions for HPLC Mobile Phase Selection
One of the early steps for method development is the selection of a mobile phase that will work best to separate your analytes. When choosing a mobile phase, selection of an appropriate buffer is one of the key factors. What is a buffer solution? A buffer…
Impact of Canning and Storage of Cartotenoids and Polyphenols in Apricots
Carotenoids are often studied in the food industry due to their nutritional value and may contribute to reduced risks of degenerative diseases like cancer [1, 2] and Parkinson’s disease. [3] Reverse phase HPLC (RP-HPLC) is a common way to identify and…